Report:
| Prof. Gwo-Bin Lee,Department of Engineering Science,National Cheng Kung University |
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Titles:
Magnetic-bead based microfluidic systems for fast diagnosis
Abstract:
In the past decade, microfabrication of miniature fluidic devices has attracted considerable interest and made substantial impacts. One of the most promising applications is for biomedical applications. In this talk, several bio-sensing chips using the enabling microfluidic technology incorporate with magnetic beads will be presented.
Functional microfluidic devices/systems have been integrated to perform fast diagnosis in an automatic format. Taking advantage of the specific interaction between the antibody on the surface of the magnetic breads and the surface antigen on the viruses or bacteria, the target viruses and bacteria can be recognized and further purified by a magnetic field.
The purified virus and bacteria were then lysed to release RNA or DNA for the subsequent RT-PCR processes. Results show that the target viruses/bacteria can be purified and concentrated successfully by the high specificity and selectivity of antibody-conjugated magnetic beads, and the subsequent amplification of RNA/DNA can be completed automatically utilizing the on-chip micro heaters.
The high mixing efficiency of the two-way s-pump and the rapid heating/cooling rate of the micro heaters can make the pretreatment and diagnosis processes in a shorter period of time. As a whole, the developed system may provide a powerful platform for sample pretreatment and fast diseases diagnosis.
Prof. Gwo-Bin Lee received his B.S. and M.S. degrees in Department of Mechanical Engineering from National Taiwan University in 1989 and 1991, respectively. He received his Ph.D. in Mechanical & Aerospace Engineering from University of California, Los Angeles, USA in 1998. Dr. Gwo-Bin Lee is currently a Distinguished Professor in the Department of Engineering Science at National Cheng Kung University. His research interests lie on nano-biotechnology, micro/nanofluidics and their biomedical applications.
He is the director of “MEMS Design and Microfabrication Lab” and “Microfluidic Biochip Lab”. Dr. Lee has been active in the field of micro/nanofluidic systems, and is developing integrated micro/nano systems incorporated with nano/biotechnology for biomedical applications. He has developed several micro/nano-scale platforms for cell, protein, and DNA manipulation and detection. Dr. Lee has published over 140 SCI journal papers, 340 conference papers, and filed 80 patents.
Time:
24th December 2009
AM 11：00-11：45
Place:
The third flood Meeting Room, Building R |
